EVALUASI SEDIAAN GARGARISMA EKSTRAK ETANOL DAUN JERUK NIPIS (Citrus aurantiifolia) TERHADAP Streptococcus mutans: EVALUASI SEDIAAN GARGARISMA EKSTRAK ETANOL DAUN JERUK NIPIS (Citrus aurantiifolia) TERHADAP Streptococcus mutans

Asfari Fauziyyah; Srie Rezeki Nur Endah; Lina Rahmawati Rizkuloh

doi:10.29313/jiff.v8i1.4927

Authors

Asfari Fauziyyah Universitas Perjuangan
Srie Rezeki Nur Endah Universitas Perjuangan
Lina Rahmawati Rizkuloh Universitas Perjuangan Tasikmalaya

DOI:

https://doi.org/10.29313/jiff.v8i1.4927

Keywords:

Antibacterial, Streptococcus mutans, Gargarisma, Lime Leaves

Abstract

The ethanol extract of lime leaves is known to have an antibacterial effect against Streptococcus mutans which causes caries, making it suitable for use in gargarisma preparations. This research aims to produce mouthwash preparations based on ethanol extract of lime leaves, and determine its activity against Streptococcus mutans bacteria. The preparation was developed with four formulas, namely F0 (base), F1 (20%), F2 (25%) and F3 (30%). Evaluation of preparations includes organoleptic tests, homogeneity tests, pH tests, specific gravity tests, and viscosity tests. The antibacterial activity test of the Gargarisma preparation used 0.2% chlorhexidine as a comparison, using the well technique. The evaluation proved that the ethanol extract of lime leaves was developed into a gargarisma preparation. The results of the activity test of the Gargarisma preparation showed an average diameter of inhibition of F0 (0 mm), F1 (15 mm), F2 (15.6 mm), F3 (16.06 mm) and positive control (14.64 mm).

Author Biographies

Asfari Fauziyyah, Universitas Perjuangan

Program Studi Farmasi, Fakultas Ilmu Kesehatan

Srie Rezeki Nur Endah, Universitas Perjuangan

Program Studi Farmasi, Fakultas Ilmu Kesehatan

Lina Rahmawati Rizkuloh, Universitas Perjuangan Tasikmalaya

Program Studi Farmasi, Fakultas Ilmu Kesehatan

References

Ajdic, D., McShan, W., Mclaughlin, R., Savić, G., Chang, J., Carson, M., Primeaux, C., Tian, R., Kenton, S., Jia, H., Lin, S., Qian, Y., Li, S., Zhu, H., Najar, F., Lai, H., White, J., Roe, B., & Ferretti, J. (2002). Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proceedings of the National Academy of Sciences of the United States of America, 99, 14434 - 14439. https://doi.org/10.1073/pnas.172501299.

Afdilla, N. A. N. (2023). “Uji Efektifitas Antibakteri Kombinasi Ekstrak Daun Kemangi (Ocimum Basilikum L) dan Daun Jeruk Nipis (Citrus Aurantifolia) Terhadap Pertumbuhan Streptococus Mutans”. Jurnal Kesehatan Dan Kesehatan Gigi, 3(2), 67-72.

Asril, W. H. (2014). Daya Hambat Minyak Atsiri Dari Daun Jeruk Nipis (Citrus Aurantifolia) Terhadap Pertumbuhan Bakteri Streptococcus Mutans (Doctoral dissertation, Universitas Andalas).

Bariyah, I., Rahayu, D., Karyus, A., Noviansyah, N., & Budiati, E. (2024). Analisis faktor yang berhubungan dengan kesehatan gigi dan mulut pada pasien skizofrenia. Jurnal Ilmiah Kesehatan Media Husada, 13(1), 34-48.

Chismirina, S., & Magistra, R. Y. (2016). Konsentrasi hambat dan bunuh minimum ekstrak daun jeruk nipis (citrus aurantifolia) terhadap aggregatibacter actinomycetemcomitans secara in vitro. Cakradonya Dental Journal, 8(1), 68-76.

Handayani, F., Warida, H., Nur, Juhairiah S. (2016). Formulasi dan Uji Aktivitas Antibakteri Streptococcus mutans dari Sediaan Mouthwash Ekstrak Daun Salam (Syzygium polyanthum (Wight) Walp,). Media Sains. Akademi Farmasi Samarinda. Vol.9, No1.

Kim, D., Ito, T., Hara, A., Li, Y., Kreth, J., & Koo, H. (2022). Antagonistic interactions by a high H2 O2 -producing commensal streptococci modulate caries development by Streptococcus mutans.. Molecular oral microbiology. https://doi.org/10.1111/omi.12394.

Kooltheat, N., Kamuthachad, L., Anthapanya, M., Samakchan, N., Sranujit, R., Potup, P., Ferrante, A., & Usuwanthim, K. (2016). Kaffir lime leaves extract inhibits biofilm formation by Streptococcus mutans.. Nutrition, 32 4, 486-90. https://doi.org/10.1016/j.nut.2015.10.010.

Kriswandini, I., & Almas, R. (2023). Review: Quorum sensing mechanism between Streptococcus mutans and Candida albicans in the pathogenesis of dental caries. World Journal of Advanced Research and Reviews. https://doi.org/10.30574/wjarr.2023.17.1.0066.

Mbawalla, H. S., Nyamuryekung’e, K., Mtaya, M., & Masalu, J.-R. (2023). Dental Caries Pattern Amongst Tanzanian Children: National Oral Health Survey. In International Dental Journal (Vol. 73, Issue 5, p. 731). Elsevier BV. https://doi.org/10.1016/j.identj.2023.03.008

Moynihan, P. (2016). Sugars and Dental Caries: Evidence for Setting a Recommended Threshold for Intake [Review of Sugars and Dental Caries: Evidence for Setting a Recommended Threshold for Intake]. Advances in Nutrition, 7(1), 149. Elsevier BV. https://doi.org/10.3945/an.115.009365

Mulyanti, S., Laela, D., Julaeha, E., Suwargiani, A., & Aripin, D. (2020). Formulation of mouth rinse from the essential oils of lime (Citrus aurantifolia) and its inhibitory efficacy on the growth of Streptococcus mutans – in vitro., 32, 39-47. https://doi.org/10.24198/pjd.vol32no1.25486.

Noor Madani, F. (2021). Aktivitas Antibakteri Ekstrak Daun Kumis Kucing (Orthosiphon Stamineus) Terhadap Bakteri Propionibacterium acnes.

Oktaviani, A. F., Rahmatullah, S., & Pambudi, D. B. (2021). Formulasi Sediaan Obat Kumur Ekstrak Etanol Daun Selasih (Ocimum Basilicum L.) Sebagai Uji Aktivitas Antibakteri Streptococcus Mutans. Jurnal Ilmiah JOPHUS : Journal Of Pharmacy UMUS, 3(01), 1–9. https://doi.org/10.46772/jophus.v3i01.518

Parama, P. W., Sukrama, I. D. M., & Handoko, S. A. (2019). Uji efektifitas antibakteri ekstrak buah jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan Streptococcus mutans in vitro. Bali Dental Journal, 3(1), 45–52. https://doi.org/10.51559/bdj.v3i1.136

Pytko-Polończyk, J., Stawarz-Janeczek, M., Kryczyk‐Poprawa, A., & Muszyńska, B. (2021). Antioxidant-Rich Natural Raw Materials in the Prevention and Treatment of Selected Oral Cavity and Periodontal Diseases [Review of Antioxidant-Rich Natural Raw Materials in the Prevention and Treatment of Selected Oral Cavity and Periodontal Diseases]. Antioxidants, 10(11), 1848. Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/antiox10111848

Rezky Yanuarty. (2021). Uji Aktivitas Antioksidan Daun Jeruk Nipis (Citrus aurantiifolia) Secara Spektrofotometri UV-Vis

Septiyanti, A. E., Retnaningsih, A., Purnama, R.C., & Al Kausar, R. (2023). Uji Aktivitas Ekstrak Daun Kelor (Moringa oleifera L) dalam sediaan Obat Kumur terhadap Candida albicans Penyebab sariawan. Jurnal Analisis Farmasi, 8 (2), 279-294.

Slamet, S., Anggun, D, B., & Pambudi, B, D., (2020). Uji Stabilitas Fisik Formula Sediaan Gel Ekstrak Daun Kelor (Moringa Oleifera Lamk.). Jurnal Ilmiah Kesehatan, Vol XIII (2), 115-122.

Wilujeng, R. I. (2022). Aktivitas antibakteri ekstrak daun jeruk nipis (citrus aurantifolia) terhadap streptococcus mutans in vitro: menggunakan metode dilusi tabung