THIN-LAYER CHROMATOGRAPHIC PROFILE AND SUNSCREEN ACTIVITY OF ETHANOL EXTRACT 70% KESUM LEAVES (Polygonum minus Huds)
PROFIL KROMATOGRAFI LAPIS TIPIS DAN AKTIVITAS TABIR SURYA EKSTRAK ETANOL 70% DAUN KESUM (Polygonum minus Huds)
DOI:
https://doi.org/10.29313/jiff.v7i2.3667Keywords:
Thin Layer Chromatography, sunscreen, kesum leafAbstract
Polygonum minus Huds. is an endemic plant in West Kalimantan. In Kalimantan, this plant is widely known by its people as one of the biological resources. Kesum has several pharmacological properties such as antimicrobial, anticancer and antioxidant activity and contain secondary metabolites such as phenols, tannins, alkaloids, and flavonoids. The purpose of this study was to identified the thin-layer chromatographic profile and sunscreen activity of 70% ethanol extract of kesum using a UV spectrophotometer. Results from this study, chromatogram profile of 70% ethanol extract of kesum leaves contained stain spots with the Rf value of each secondary metabolite, namely: alkaloids (Rf = 0.72), flavonoids (Rf = 0.64), saponins (Rf = 0.54), tannins (Rf = 0.75), and phenols (Rf = 0.47). The results of sunscreen activity testing obtained the highest SPF (Sun Protection Factor) value of 15.23, the lowest % Erythema Transmission value of 1.53% and the lowest % Pigmentation Transmission value of 6.53%. 70% ethanol extract of chem leaves has high potential as a sunscreen.
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