ANALISIS REGION TRANSMEMBRAN PROTEIN L1 HPV TIPE 52 ISOLAT INDONESIA SECARA IN-SILICO: ANALISIS REGION TRANSMEMBRAN PROTEIN L1 HPV TIPE 52 ISOLAT INDONESIA SECARA IN-SILICO

HENIK ANJAYATI

doi:10.29313/jiff.v7i1.3195

Authors

HENIK ANJAYATI ITBK-MT, MTs YUSUF ANNUR

DOI:

https://doi.org/10.29313/jiff.v7i1.3195

Keywords:

Hydrophobic, L1 HPV 52, Transmembrane region

Abstract

The transmembrane region is the region of a protein that is inserted into the cell and has a hydrophobic tendency. One of these regions can be found in the L1 protein, which is a protein that makes up the human papillomavirus (HPV) icosahedral capsid. HPV (Human Papillomavirus) is a virus causing cervical cancer both nationally and globally. Cervical cancer is the third main cause of cancer in women globally and the first main cause in Indonesia. One of the most common types of HPV causing cervical cancer in Indonesia is HPV type 52, with the highest prevalence of around 23.2%. Development of a VLP-based vaccine using L1 HPV type 52 isolated from Indonesia is needed. VLP was the result of L1 protein assembly. However, L1 protein has a transmembrane region that is mostly hydrophobic and will usually form an aggregate. it will cause the targeted protein to settle in the water. Therefore, in this study, an analysis of the transmembrane region of the L1 HPV type 52 protein was carried out which had undergone cleavage of several bases at the N-terminal and C-terminal. The results showed that the recombinant L1 HPV type 52 protein, which is a truncated segment, did not have a transmembrane region when compared to the native L1 HPV type 52 protein.

Keyword: Hydrophobic, L1 HPV 52, and Transmembrane region

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