PENETAPAN KADAR FLAVONOID DAN PENGARUH EKSTRAK BIJI KARIKA TERHADAP JUMLAH LEUKOSIT PADA MENCIT YANG DIINDUKSI KARAGENAN : PENETAPAN KADAR FLAVONOID DAN PENGARUH EKSTRAK BIJI KARIKA TERHADAP JUMLAH LEUKOSIT PADA MENCIT YANG DIINDUKSI KARAGENAN

Fania Putri Luhurningtyas; Mir-a Kemila; Suzan Astyamalia; Evi Novitasari

doi:10.29313/jiff.v7i2.2855

Authors

Fania Putri Luhurningtyas Universitas Tidar
Mir-a Kemila Universitas Tidar
Suzan Astyamalia Universitas Tidar
Evi Novitasari Universitas Ngudi Waluyo

DOI:

https://doi.org/10.29313/jiff.v7i2.2855

Keywords:

karagenan, biji karika, flavonoid, leukosit

Abstract

Carica seeds (Carica pubescens) are herbs that have anti- Carica pubescens seeds are herbal remedies that have anti-inflammatory effects. The parameter for anti-inflammatory activity can be assessed by measuring the leukocyte count in test animals. This study aimed to measure the flavonoid content and the impact of various Carica pubescens seed ethanol extract doses on leukocyte count in animals induced with carrageenan. The secondary metabolites of Carica pubescens seeds were extracted using the maceration method with 70% ethanol as the solvent. The measurement of flavonoid levels was determined using routine standards through UV-visible spectrophotometry. The leukocyte count measurement method used Swiss-Webster strain mice, divided into six treatment groups: positive control, sick or negative control, solvent control, and extract doses of 100, 200, and 400 mg/kgBW. Inflammation was induced using carrageenan solution, and the leukocyte count was determined at 0, 3, and 6 hours. The results showed that Carica pubescens seed ethanol extract doses of 200 mg/kgBW and 400 mg/kgBW had an effect in reducing the leukocyte count in mice at 6 hours after carrageenan induction. This effect is influenced by the presence of flavonoid secondary metabolites. The research results obtained the flavonoid levels of Carica pubescent seed ethanol extract equivalent to routine, which is 1.346 mg/gram.

Author Biographies

Mir-a Kemila, Universitas Tidar

Program Studi Agroteknologi, Fakultas Pertanian

Suzan Astyamalia, Universitas Tidar

Program Studi Agroteknologi, Fakultas Pertanian

Evi Novitasari, Universitas Ngudi Waluyo

Program Studi Farmasi, Fakultas Kesehatan

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